Published 2014 | Version v1
Dataset Open

Vibrio Yi-Wei style

  • 1. ROR icon California Institute of Technology

Description

Strain MKW1383 grown in conditions Yi-Wei uses for Bdellovibrio predation

Technical info

Tilt Series Date: 2014-02-21

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Grown 24 hours in LB at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew at 30C for another 16 hours

aba2014-02-21-3_zap3728.jpg: Vibrio cholerae long polar-associated array (~36nm)

Attached Files

VibrioYiWeiStyle0003.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioYiWeiStyle0003_full.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB

Files

aba2014-02-21-3_zap3728.jpg

Files (3.9 GB)

Name Size
md5:a1b6a5785bf94f966ee402046c4d7506
435.6 kB Preview Download
md5:c0180fbc97ea4c98e303414ee36e7f64
1.1 MB Preview Download
md5:538ddcf0ac4d0e03f4f446ed6f61a6e9
17.6 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/27dqy-gkm62
TILT_SERIES_ID
aba2014-02-21-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-02-21
Created
2014-02-21
Updated
2014-09-24