O. tauri MG132 arrest

Description

Technical info

Tilt Series Date: 2009-11-16

Data Taken By: Lu Gan

Species / Specimen: Ostreococcus tauri

Strain: RCC745

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132

Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF

Attached Files

RCC745_MGtovis_414.mrc, Tilt Series (Pixel Size 1.26 nm), 746.6 MB

RCC745_MGtovis_414.rec, Reconstruction (Pixel Size 1.26 nm), 558.6 MB