Xanthomonas vs Caulobac (-T1SS)

Description

Technical info

Tilt Series Date: 2017-10-21

Data Taken By: Debnath Ghosal

Species / Specimen: Xanthomonas citri

Strain: pv citri 306

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Chuck Farah lab made the Xac strain, Caulobacter strain is from Laub lab. Data collected and processed by Mohammed Kaplan and Debnath Ghosal.

Purification / Growth Conditions / Treatment: 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.

Sample Preparation: Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot manually.

Attached Files

Xan_CaulDelT1_20172110f0003.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB

Xan_CaulDelT1_20172110f0003.rec, Reconstruction (Pixel Size 1.56 nm), 889.0 MB