Published 2015 | Version v1
Dataset Open

Cos7 cells

  • 1. ROR icon California Institute of Technology

Description

Looking for midbodies or Tunelling nanotubes

Technical info

Tilt Series Date: 2015-03-05

Data Taken By: Rasika Ramdasi

Species / Specimen: Cos7

Strain: Cos7 cells

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF TOMO

Sample Preparation: Synchronized cells with Thymidine 2mM. Frozen after 14 hours of washing.

rra2015-03-05-1_slicer12803.jpg: ESCRT repeating units? rra2015-03-05-1_slicer12804.jpg: Thinner filaments rra2015-03-05-1_slicer12807.jpg: Repeating units in ESCRT(?) filament.. spacing- 3 nm rra2015-03-05-1_slicer12808.jpg: Thin filaments around midbody

Attached Files

atlas10001.mrc, Tilt Series (Pixel Size 0.5 nm), 2.3 GB

atlas10001_part81_2.rec, Reconstruction (Pixel Size 2.0 nm), 623.6 MB

atlas10001new.st_full.rec, Reconstruction (Pixel Size 0.0 nm), 311.1 MB

Files

keyimg_rra2015-03-05-1.jpg

Files (3.3 GB)

Name Size
2.3 GB (external) Download
623.6 MB (external) Download
311.1 MB (external) Download
md5:06da131ed60562f45fd27fe08e05bb65
1.1 MB Preview Download
md5:1fe583e4dfb5a9202150dd0a67ad84be
21.5 MB Preview Download
md5:ad8eb6330c544fd6728863fa7a5f4880
231.6 kB Preview Download
md5:fd37fe0884a2ac4aa513ef70841b1e40
398.9 kB Preview Download
md5:034113cbd88ef230a50c7e24da8e2668
381.2 kB Preview Download
md5:856ffbe42d238ba7662ec51f0aac24d1
168.5 kB Preview Download
md5:e2a3f383865f376a0dc81c29673b16a2
429.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/2djch-pr609
TILT_SERIES_ID
rra2015-03-05-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-05
Created
2015-03-05
Updated
2015-03-11