Hylemonella gracilis

Description

Technical info

Tilt Series Date: 2006-10-20

Data Taken By: Gavin Murphy

Species / Specimen: Hylemonella gracilis

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 60.0°), step: 0.9°, constant angular increment, dosage: 75.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: Cells provided by Jared Leadbetter; Data collected and processed by Gavin Murphy

Purification / Growth Conditions / Treatment: Grown in dilute media without salt. 0.5 g/L of Tryptone, 0.5 g/L YE. Cells grown for 2 days. OD never gets above 0.005. Centrifuged lightly e.g. 3500x g, then concentrated 50-100x fold.

Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...

H20_061020_tilt.jpg: * associated to rawdata H20_061020_rec.jpg: file associated to 3D Image #115 zap000.jpg: file associated to 3D Image #115

Attached Files

A20_S474G4_34k12d75e4nm.mrc, Tilt Series (Pixel Size 0.67 nm), 1.2 GB

A20NoCTFrec0.28.mrc, Reconstruction (Pixel Size 0.0 nm), 170.5 MB

A20NoCTFhigh_44_part.pif, Reconstruction (Pixel Size 0.0 nm), 2.7 MB

zap000.tif, file associated to 3D Image #115, 1.5 MB