O. tauri Propyzamide release

Description

Technical info

Tilt Series Date: 2010-02-12

Data Taken By: Lu Gan

Species / Specimen: Ostreococcus tauri

Strain: RCC745

Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide

Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF

lga2010-02-12-160_slicer5664.jpg: stacked membrane . Unknown

Attached Files

RCC745_RELtovis_172.mrc, Tilt Series (Pixel Size 1.26 nm), 562.0 MB

RCC745_RELtovis_172.rec, Reconstruction (Pixel Size 1.26 nm), 345.4 MB