Sulfolobus solfataricus

Description

Technical info

Tilt Series Date: 2010-01-04

Data Taken By: Lu Gan

Species / Specimen: Sulfolobus solfataricus

Strain: 2-1-12

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: None μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Prepared sample: Chi-yu FuDesigned experiments: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. JohnsonCollected data: Chi-yu Fu, Lu GanAnalyzed data: Chi-yu Fu, Kang Wang, Lu Gan, Jason Lanman, Reza Khayat, Mark J. Young, Grant J. Jensen, Peter C. Doerschuk, John E. Johnson

Purification / Growth Conditions / Treatment: S. solfataricus strain 2-2-12 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~22 at OD 650 nm ~0.4. At 22–32 hr postinfection, cells were concentrated ~20 fold by centrifugation.

Sample Preparation: Equal volume of cells were mixed with BSA-treated 10 nm gold colloidal beads and applied to plasma cleaned Quantifoil holey carbon films. The grids were plunge-frozen in liquid ethane using a Vitrobot (FEI Inc.) and stored in liquid nitrogen.

Attached Files

26.mrc, Tilt Series (Pixel Size 1.26 nm), 1.0 GB

26_full.rec, Reconstruction (Pixel Size 2.52 nm), 629.1 MB