MS411-R1-16 / WM1652
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-04-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strains from Carrie Shaffer
Purification / Growth Conditions / Treatment: MS411-R1-16 & WM1652 grew to OD 0.6 in LB. Mix 2.5 ul of each onto grids on LB-agar plate, incubate 30 min for conjugation.
Sample Preparation: Vitrobot settings: 100% humidity, 22.5 C, 3µl BSA treated 10 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 1 filter paper for 3 sec. Ethane/propane mix.
ycw2014-04-07-1_slicer6963.jpg: Peptidoglycan ycw2014-04-07-1_slicer6965.jpg: filament
Attached Files
20140407_YWC095_10001.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
20140407_YWC095_10001_part131_2.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2014-04-07-1.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:c156da0cded4f603c43036cd9317fcd1
|
1.0 MB | Preview Download |
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md5:55c4a3ca5cad87670b19a7ce6d941a3c
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22.6 MB | Preview Download |
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md5:389af8ec9ce6be0e25b2336b1181f44f
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74.6 kB | Preview Download |
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md5:7c9b150eac9904069d64e2292d4cb019
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76.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/2hyq3-bcc95
- TILT_SERIES_ID
- ycw2014-04-07-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-04-07
- Created
-
2014-04-07
- Updated
-
2014-07-15