Published 2011
| Version v1
Dataset
Open
E. coli (pLE7, 20 uM IPTG)
Description
Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.
Technical info
Tilt Series Date: 2011-06-02
Data Taken By: Matt Swulius
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFTomo
Collaborators and Roles: Gift from Larry Rothfield
Purification / Growth Conditions / Treatment: Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.
Sample Preparation: Vitrobot plunge freezing
Attached Files
20110602_PLE7_09.mrc, Tilt Series (Pixel Size 0.95 nm), 1.0 GB
20110602_PLE7_09_full.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB
Files
keyimg_mst2011-06-02-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/2mn3b-bjh42
- TILT_SERIES_ID
- mst2011-06-02-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-06-02
- Created
-
2012-07-03
- Updated
-
2012-07-05