Myxococcus xanthus pilM-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-05-16
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-05-16-13_slicer6890.jpg: External Vesicle ycw2014-05-16-13_slicer6891.jpg: Peptidoglycan
Attached Files
20140516_Janelia_YWC096_10038.rec, Reconstruction (Pixel Size 1.32 nm), 1.4 GB
20140516_Janelia_YWC096_10038.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
Files
keyimg_ycw2014-05-16-13.jpg
Files
(5.0 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 1.4 GB | (external) Download | |
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md5:cb14e35dc82f45d44e70e03df242f47d
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988.4 kB | Preview Download |
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md5:0e9382f072c267a56d5740d45e80f199
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27.9 MB | Preview Download |
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md5:9d0c256ada9aa4755dc6af5b5c60aeff
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203.7 kB | Preview Download |
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md5:30cfe1877a4f2bb4816b949cffa31acf
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230.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/2qt50-54b24
- TILT_SERIES_ID
- ycw2014-05-16-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-05-16
- Created
-
2014-05-19
- Updated
-
2014-07-15