Legionella KS79 (WT), 42 C_On

Description

Technical info

<strong>Tilt Series Date:</strong> 2019-05-28</p>

<strong>Data Taken By:</strong> Debnath Ghosal</p>

<strong>Species / Specimen:</strong> Legionella pneumophila</p>

<strong>Strain:</strong> Philadelphia-1_Lp02</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 42000x. </p>

<strong>Microscope:</strong> Janelia Titan</p>

<strong>Acquisition Software:</strong> UCSFTomo</p>

<strong>Upload Method:</strong> pipeline</p>

<strong>Processing Software Used:</strong> Raptor</p>

<strong>Collaborators and Roles:</strong> Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.</p>

<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>

External Files

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