Dividing Caulobacter

Description

Technical info

Tilt Series Date: 2015-03-10

Data Taken By: Qing Yao

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 180/A2. defocus: -10um. magnification: 27500.

qya2015-03-10-29_slicer12963.jpg: Invagination on both sides qya2015-03-10-29_slicer12964.jpg: low image quality

Attached Files

Qing15_10029.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Qing15_10029_part121_56.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB