Myxococcus xanthus TM530
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-10-25
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Tam Mignot
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200
ycw2013-10-25-22_slicer10555.jpg: Myxococcus xanthus T6SS with additional helical filament layer
Attached Files
20131025_YWC029_20004.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
20131025_YWC029_20004_part121_6.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2013-10-25-22.jpg
Additional details
Identifiers
- DOI
- 10.22002/35aym-dda22
- TILT_SERIES_ID
- ycw2013-10-25-22
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-10-25
- Created
-
2013-10-26
- Updated
-
2014-11-03