Published 2005 | Version v1
Dataset Open

Caulobacter crescentus

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

wild type CB15N. 2 of 6, not processed. 'skinny' cells; low contrast tilt series

Technical info

Tilt Series Date: 2005-11-30

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.2°, 60.2°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software:

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep &data collection: D. Prabha Dias (not processed)

Purification / Growth Conditions / Treatment: M2M media (RB Jensen protocol); cultured in 50ml flask; exponential phase; see DPD notebook Nov 21-28, 2005

Sample Preparation: box 506, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -1, blot 1 sec, drain 1 sec; see DPD notebook Nov 28, 2005

Attached Files

CC05Nov30t02.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB

SAVE_05Nov29t02.tif, 4.4 MB

SAVE_CC05Nov30t02_13kx.dm3, 8.6 MB

Files

keyimg_pdd2005-11-30-2.jpg

Files (1.5 GB)

Name Size
1.5 GB (external) Download
md5:bbb668c015fc7f44680e333915659801
3.1 MB Preview Download
md5:dfe57a84b591044b78ccbaae4e67fd0f
140.3 kB Preview Download
4.4 MB (external) Download
8.6 MB (external) Download

Additional details

Identifiers

DOI
10.22002/35rvg-75w78
TILT_SERIES_ID
pdd2005-11-30-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2005-11-30
Created
2008-06-18
Updated
2008-06-11