Myxococcus xanthus delta-pilB
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-12-31
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.
ycw2013-12-31-10_slicer8964.jpg: Filament bundle . Myxococcus xanthus ycw2013-12-31-10_slicer8976.jpg: Filament Bundle . Myxococcus xanthus
Attached Files
20131231_YWC066_10010.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20131231_YWC066_10010_part131_20.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2013-12-31-10.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
|
md5:c6234d4784745c90ebad0cee28ba25e1
|
1.1 MB | Preview Download |
|
md5:80869d3fa1d971a64a6d3e3b9e11d75b
|
23.2 MB | Preview Download |
|
md5:ac4ab03f72f9d925c79a12af80079a59
|
352.3 kB | Preview Download |
|
md5:7c6bdd1be4ecc7d25ba325c7d2a65e9c
|
421.9 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/370tj-ysr48
- TILT_SERIES_ID
- ycw2013-12-31-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-12-31
- Created
-
2014-01-01
- Updated
-
2014-07-30