H. pylori 26695 WT / AGS

Description

Technical info

Tilt Series Date: 2017-02-27

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain and sample prepared by Carrie Shaffer

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 10 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.

ycw2017-02-27-16_slicer17860.jpg: cag T4SS wi fi particle ycw2017-02-27-16_slicer17861.jpg: cag T4SS wi fi particle ycw2017-02-27-16_slicer17862.jpg: partially assembled cag T4SS wi fi particle? ycw2017-02-27-16_slicer17863.jpg: T4SS channel/tube? ycw2017-02-27-16_slicer17864.jpg: cag T4SS pilus/channel? . . cag T4SS (green)

Attached Files

20170227_YWC243_10015.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB

20170227_YWC243_10015_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB