Helicobacter pylori G27

Description

Technical info

Tilt Series Date: 2012-11-14

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: G27

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Strain form Din Jing, NIH

Purification / Growth Conditions / Treatment: Cells grown in Brucella broth (Fluka) + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.3

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.

Attached Files

Hpylori_0020008.mrc, Tilt Series (Pixel Size 0.49 nm), 2.3 GB

Hpylori_0020008_full.rec, Reconstruction (Pixel Size 1.96 nm), 441.7 MB