Published 2014
| Version v1
Dataset
Open
Hippocampal Neurons (cultured)
- Researchers:
- Zhuang, Xiawei
- Zhou, Ruobo
Citation
APA
Description
Plunge frozen at DIV 14
Files
keyimg_mst2014-08-21-16.jpg
Technical info
Tilt Series Date: 2014-08-21
Data Taken By: Matt Swulius Species / Specimen: Rat Hippocampal Neuron (cultured) Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging) Purification / Growth Conditions / Treatment: Plunge frozen at DIV 14Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing. Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.External Files
Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-08-21-16
082014_neurons0027.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB Download 082014_neurons0027.rec, Reconstruction (Pixel Size 2.4 nm), 1.4 GB DownloadAdditional details
- TILT_SERIES_ID
- mst2014-08-21-16
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2014-08-21
- Created
-
2014-08-21
- Updated
-
2014-08-24