Lp02 ΔpilQ

Description

Technical info

Tilt Series Date: 2017-03-02

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Materials Sciences F30

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor, also manually processed

Collaborators and Roles: Strain sent to us by Nicholas Cianciotto at Northwestern University Medical SchoolData collected and processed by Debnath Ghosal

Sample Preparation: 4 grids were frozen for Lp02 at OD= 2.8 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

Attached Files

delta_Q0018.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB

delta_Q0018_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB