Published 2009 | Version v1
Dataset Open

Hyphomonas EB session21 34KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-07-14

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: raptor

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in PYE media, EB 0.02mg/ml overnight 4degree...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

Attached Files

09jul14a_b200c_081.mrc, Tilt Series (Pixel Size 0.632 nm), 520.1 MB

09jul14a_b200c_081_part.rec, Reconstruction (Pixel Size 1.264 nm), 367.0 MB

Files

keyimg_jsa2009-07-14-80.jpg

Files (899.4 MB)

Name Size
520.1 MB (external) Download
367.0 MB (external) Download
md5:a568faba92793d00260c4749553dcddd
1.3 MB Preview Download
md5:014a61d123dc987f5a2ae20abcb62baa
11.1 MB Preview Download

Additional details

Identifiers

DOI
10.22002/3pnpj-f5855
TILT_SERIES_ID
jsa2009-07-14-80

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-07-14
Created
2009-10-20
Updated
2009-10-20