Published 2020
| Version v1
Dataset
Open
pl_scale5
Description
Raw data files of pl_scale5
Files
keyimg_wnj2020-11-09-14.jpg
Technical info
Tilt Series Date: 2020-11-09
Data Taken By: William Nicolas Species / Specimen: Allium cepa Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x. Microscope: Caltech Titan Krios Acquisition Software: Serial EM Upload Method: webload Processing Software Used: IMOD Purification / Growth Conditions / Treatment: Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing Sample Preparation: Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-11-09-14/rawdata/
on29-2_lam5_ts1.mrc, Tilt Series (Pixel Size 0.33 nm), 1.4 GB Download on29-2_lam5_ts1_rec.hdf, Reconstruction (Pixel Size 1.32 nm), 1.2 GB Download postprocess_masked_on29.mrc, Reconstruction (Pixel Size 0.67 nm) Average done by Przchemek, 257.0 kB Download on29-2_lam5_ts1.rawtlt, 240.0 B Download on29-2_lam5_ts1.mrc.mdoc, 17.6 kB DownloadAdditional details
- DOI
- 10.22002/3qb34-qzr07
- TILT_SERIES_ID
- wnj2020-11-09-14
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2020-11-09
- Created
-
2022-03-29
- Updated
-
2022-04-04