Behavioral and neuronal changes after chronic loss of inhibition in a pre-motor avian circuit

Description

Mastering essential motor skills is crucial for an animal's survival and reproduction, enabling it to endure diverse perturbations throughout its lifespan, such as trauma, disease, and aging. What mechanisms orchestrate brain circuit reorganization and recovery to maintain stability of behavioral output during the continued presence of a disturbance? In this study, we investigate the consequences at the behavioral, neuronal, and transcriptome level of chronically perturbing a complex behavior over time. Our electrophysiology recordings suggest a relationship between offline activity dynamics and circuit restoration for behavior. Furthermore, single-cell RNA sequencing reveals cellular mechanisms underlying behavioral recovery suggesting a role for synaptic plasticity in this restoration process mediated by microglia and MHC class I. Our findings offer compelling evidence that the adult brain has the ability to surmount prolonged circuit perturbations through the utilization of offline activity and repair mediated by microglia.

This dataset contains the last file belongs to the first control animal (starting with SE_) from one animal left over from the single-cell RNA sequencing files uploaded with DOI: 10.22002/ednra-nn006. 

The following dataset that contains .wav files pertain to the songs the animals produced after either control or interneuron muting virus injection into the pre-motor nucleus of adult male zebra finches. This is the first chronic manipulation of the interneuron population in this pre -motor circuit, which surprisingly is a large-scale perturbation the system can overcome within 60-90 days after loss of precise execution of behavior for about 40-80 days. 

The last dataset that contains .mat files pertains to the electrophysiology, sounds and head-movement data collected during chronic electrophysiology recordings either in control or interneuron muted animals. This is the first dataset of this type to be recorded. 

Please contact us with any questions, comments or concerns: szofitorok8@gmail.com , zsofi@caltech.edu, lauraluebbert@caltech.edu, clois@caltech.edu

Files

Technical info

The .mat files that start with B138 are of a chronically implanted animal's sound, head-movement and electrophysiology data with muting of interneurons in HVC. The .mat files with ID PK31 are of a control animal with a similar implant.  The .zip file contains sound recordings for animals that we performed acute head fixed electrophysiology. IDs of animals label folders in which you find .wav files that were curated at different times during the perturbation. V654, V652 are control animals with normal song, while V664,V668,V670,V671 animals injected with interneuron muting virus recorded for electrophysiology at 3-6 days post injection; V647,V648 are animals injected with interneuron muting virus recorded at 20 days post injection and at 70 days post injection ( when their song recovered) for electrophysiology.