Published 2011 | Version v1
Dataset Open

HUVECs CHMP4A+VPS4DN

  • 1. ROR icon California Institute of Technology

Description

HUVECs cells transfected with CHMP4A+VPS4DN constructs using Nucleofector Kit. Sample# C58-2

Technical info

Tilt Series Date: 2011-03-31

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: CHMP4A and VPS4DN construct provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected

Sample Preparation: manual blotting

Attached Files

11mar31a_C_58-2_025_full.rec, Reconstruction (Pixel Size 2.0 nm), 478.2 MB

11mar31a_C_58-2_025.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

11mar31a_C_58-2_025_full_ring?.tiff, file associated to 3D Image #68441, 1.0 MB

Files

11mar31a_C_58-2_025_full_ring?.tiff

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Files (1.5 GB)

Name Size
1.0 GB (external) Download
478.2 MB (external) Download
1.0 MB (external) Download
md5:2952b4b9310b9fedc8c6d7248cabe72e
1.2 MB Preview Download
md5:79433a2e6ced343259d0b94999c6e741
7.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/3ybm6-cff36
TILT_SERIES_ID
cwl2011-03-31-21

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-03-31
Created
2015-09-16
Updated
2015-09-16