Published 2010 | Version v1
Dataset Open

L256T chemotaxis mutant

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

L256T HAMP mutant locked in an 'CheA activating state'

Technical info

Tilt Series Date: 2010-03-18

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Acquisition Software: UCSF Tomo

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: John Parkinson

Purification / Growth Conditions / Treatment: grown in TB media with Chloramphenicol, induced with 0.6µM salicylate for 3 hours, penicillin treated for 1 hour

Attached Files

10mar29a_EcoliL256T_026.st, Tilt Series (Pixel Size 0.632 nm), 1.0 GB

10mar29a_EcoliL256T_026.rec, Reconstruction (Pixel Size 0.632 nm), 335.0 MB

Files

keyimg_aba2010-03-18-35.jpg

Files (1.4 GB)

Name Size
335.0 MB (external) Download
1.0 GB (external) Download
md5:ca16ad1f9139759e9b6ec301b29d89e3
785.1 kB Preview Download
md5:c57e8b3ce6cdd9c6d3e8e7c9f0cfab00
17.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/3zqcv-hrp77
TILT_SERIES_ID
aba2010-03-18-35

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2010-03-18
Created
2010-07-12
Updated
2010-07-12