Myxococcus xanthus PilO-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20140306_YWC092_10010.mrc, Tilt Series (Pixel Size 4.2 nm), 3.6 GB
20140306_YWC092_10010_full.rec, Reconstruction (Pixel Size 16.8 nm), 688.9 MB
Files
keyimg_ycw2014-03-07-5.jpg
Additional details
Identifiers
- DOI
- 10.22002/41dqn-v5g04
- TILT_SERIES_ID
- ycw2014-03-07-5
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-07
- Created
-
2014-03-08
- Updated
-
2014-03-17