Published 2015 | Version v1
Dataset Open

COS7 CHMP4B+VPS4DN

  • 1. ROR icon California Institute of Technology

Contributors

Description

Cos7 cells transfected with CHMP4B & VPS4DN constructs

Technical info

Tilt Series Date: 2015-02-07

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with Lipofectamine

Sample Preparation: blotting

Attached Files

15feb08_c208-1_AT1BT1-0003.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB

15feb08_c208-1_AT1BT1-0003_full.rec, Reconstruction (Pixel Size 2.0 nm), 534.5 MB

Files

keyimg_cwl2015-02-07-9.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:d0aa4506c44e68d2bb1fb1d74eac557a
1.0 MB Preview Download
md5:5aa4f95855d9595867f245fc4ed27c71
20.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/45sdr-8nw73
TILT_SERIES_ID
cwl2015-02-07-9

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-02-07
Created
2015-02-09
Updated
2019-03-07