Published 2017 | Version v1
Dataset Open

T5_wt_control_Aumalemaide_5nm

  • 1. ROR icon California Institute of Technology

Description

This is a wt sample in which Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. This is a control sample. No fiducials added.

Technical info

Tilt Series Date: 2017-02-14

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain: EC93 stick, UPEC536

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: autoPatch

Collaborators and Roles: Zach Ruhe and Chris hayes

Purification / Growth Conditions / Treatment: Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.

Sample Preparation: Sample prepared by Taylor Steven

Attached Files

sq90011.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB

sq90011.rec, Reconstruction (Pixel Size 1.56 nm), 1.1 GB

Files

keyimg_psa2017-02-14-11.jpg

Files (4.8 GB)

Name Size
md5:4904fd6d0bd1b8017531ee3268feb0b2
1.0 MB Preview Download
md5:783d11b043314223db7de4f0f2eab743
16.6 MB Preview Download
3.7 GB (external) Download
1.1 GB (external) Download

Additional details

Identifiers

DOI
10.22002/45zgp-xjh67
TILT_SERIES_ID
psa2017-02-14-11

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-02-14
Created
2017-02-15
Updated
2019-03-25