Helicobacter pylori 26695
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-10-25
Data Taken By: Yi-Wei Chang
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Cell prepared by Lee Rettberg.
Purification / Growth Conditions / Treatment: Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200
Attached Files
20131025_Janelia_YWC043_20034.mrc, Tilt Series (Pixel Size 0.42 nm), 2.8 GB
20131025_Janelia_YWC043_20034_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2013-10-25-88.jpg
Additional details
Identifiers
- DOI
- 10.22002/46wpb-pda10
- TILT_SERIES_ID
- ycw2013-10-25-88
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-10-25
- Created
-
2013-10-28
- Updated
-
2013-11-05