Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

V.cholerae

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.

Technical info

Tilt Series Date: 2017-01-06

Data Taken By: Poorna Subramanian

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Yi-wei

Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.

psa2017-01-06-105_slicer17560.jpg: coated vesicles psa2017-01-06-105_slicer17561.jpg: double membrane vesicle psa2017-01-06-105_slicer17562.jpg: filaments psa2017-01-06-105_slicer17563.jpg: connected vesicles psa2017-01-06-105_slicer17564.jpg: ribisome exclusion zone

Attached Files

sq110013_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB

sq110013.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Files

keyimg_psa2017-01-06-105.jpg

Files (4.2 GB)

Name Size
md5:de6f485c9298353a6c1da408fdf69c7d
1.1 MB Preview Download
md5:6f88e0fbbe07c7dc00d5628f7404502d
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561.4 kB Preview Download
md5:701b14be4669043758d0df32830cce19
631.4 kB Preview Download
md5:580e2f5c1d2eda46f85b00a63bd19dac
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md5:6af042e79ba173a6792aac0e839d1270
487.9 kB Preview Download
md5:ea29f20b6d45c1a612356e7bb351bf7b
423.0 kB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/476s2-zg918
TILT_SERIES_ID
psa2017-01-06-105

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-01-06
Created
2017-01-10
Updated
2017-01-24