Published 2014
| Version v1
Dataset
Open
E. Coli A20S
Contributors
Researcher:
Description
Mutant MreB with sfGFP sandwich fusion.
Technical info
Tilt Series Date: 2014-04-06
Data Taken By: Matt Swulius
Species / Specimen: Escherichia coli
Strain: A20S
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: raptor
Collaborators and Roles: Josh Schaevitz
Purification / Growth Conditions / Treatment: 37 degrees celsius to OD 600 ~ 0.3
Sample Preparation: Plunge frozen in vitrobot with 100% humidity using manual blotting from rear.
Attached Files
040614_a20s_0002.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
040614_a20s_0002.rec, Reconstruction (Pixel Size 1.92 nm), 1.8 GB
Files
keyimg_mst2014-04-06-10.jpg
Additional details
Identifiers
- DOI
- 10.22002/47g0p-fe054
- TILT_SERIES_ID
- mst2014-04-06-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-04-06
- Created
-
2014-04-14
- Updated
-
2014-04-14