Published 2014 | Version v1
Dataset Open

E. Coli A20S

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Mutant MreB with sfGFP sandwich fusion.

Technical info

Tilt Series Date: 2014-04-06

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain: A20S

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: raptor

Collaborators and Roles: Josh Schaevitz

Purification / Growth Conditions / Treatment: 37 degrees celsius to OD 600 ~ 0.3

Sample Preparation: Plunge frozen in vitrobot with 100% humidity using manual blotting from rear.

Attached Files

040614_a20s_2_0001.mrc, Tilt Series (Pixel Size 0.48 nm), 1.9 GB

040614_a20s_2_0001.rec, Reconstruction (Pixel Size 1.92 nm), 1.8 GB

Files

keyimg_mst2014-04-06-13.jpg

Files (3.7 GB)

Name Size
1.9 GB (external) Download
1.8 GB (external) Download
md5:4b3ef54fac6fcc971d008c6830af4e33
1.0 MB Preview Download
md5:f9466de656a9bbd088e44149eb898ee8
4.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/49pjw-pe116
TILT_SERIES_ID
mst2014-04-06-13

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-06
Created
2014-04-14
Updated
2014-04-14