Published 2019 | Version v1
Dataset Open

Conjugation_Ecoli

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

These are conjugation samples in Ecoli. The donor strain is ANB35 and the recipient strain is . ANB24. More information on these strains can be found in the paper (Direct visualization of . horizontal gene transfer). These are tomograms from (Square 1) in Grid 1 from box 4 (see . William's notes for more information).

Technical info

Tilt Series Date: 2019-06-23

Data Taken By: Mohammed Kaplan

Species / Specimen: Escherichia coli

Strain: ANB35, Hfr3000 mrfp1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: None

Collaborators and Roles: The samples were sent by Ariel B Lindner (who is the second author on the above-mentioned . paper) in Paris.

Attached Files

AnchorLM.mrc, Tilt Series (Pixel Size 0.449 nm), 153.2 MB

AnchorLM.mrc.mdoc, 6.8 kB

Files

keyimg_mka2019-06-23-3.jpg

Files (158.3 MB)

Name Size
153.2 MB (external) Download
6.8 kB (external) Download
md5:f39cd4bc1645f2b95e33d0cc87677c15
5.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/4aa1j-4t893
TILT_SERIES_ID
mka2019-06-23-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2019-06-23
Created
2019-06-25
Updated
2022-10-15