Published 2013
| Version v1
Dataset
Open
gold labeled CheA- in vitro
Description
post wash sample - gold labeled CheA, 1.8 nm gold, post ternary formation snap rxn.
Technical info
Tilt Series Date: 2013-03-22
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 41000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Heather Hodge and Laura Kiessling
Purification / Growth Conditions / Treatment: Buffer: 1x Snap + 1mM DTT 50 mM Tris Hcl pH 7.5, 100 mM Ncl, 0.1% Tween-200)
Attached Files
HTH1B_10012.mrc, Tilt Series (Pixel Size 0.2989 nm), 1.8 GB
HTH1B_10012_part67_2.rec, Reconstruction (Pixel Size 0.5978 nm), 2.8 GB
Files
keyimg_aba2013-03-22-58.jpg
Additional details
Identifiers
- DOI
- 10.22002/4ag8r-k4s55
- TILT_SERIES_ID
- aba2013-03-22-58
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-03-22
- Created
-
2013-05-06
- Updated
-
2013-06-05