V. cholerae RT4236 (refreshed)

Description

Technical info

Tilt Series Date: 2015-09-09

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: O395-N1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain from Ronald K. Taylor

Purification / Growth Conditions / Treatment: V. cholerae 0395-N1 pMT5 grown in 5ml LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin in glass tube shaking for overnight. After took the tube out from shaker, supernatant was removed and pellet was resuspended by gentle pipetting in 1ml fresh LB without IPTG nor ampicillin. Culture was incubated at room temperature for ~15 min before mixing with gold and freezing.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 3.5 µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

Attached Files

20150909_AK_pMT15_40020.rec, Reconstruction (Pixel Size 1.56 nm), 1.1 GB

20150909_AK_pMT15_40020.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB