perfusiondcrp_fixd_good track

Description

Technical info

Tilt Series Date: 2015-08-24

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: The sample was grown in perfusion chamber and fixed for 30 minutes with 4% formaldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1.5ul gold beads.

Purification / Growth Conditions / Treatment: Lm is taken before and after fixing. In the LM after fixing the nanowires were shrunk. Although the cells looked good with formaldehyde we had lost long nanowires. 2 squares were imaged with correlation anyway.

Attached Files

perfusiondcrp_sq170005_part121_30.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

perfusiondcrp_sq170005.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB