Caltech Tomography Archive
Published 2012 | Version v1
Dataset Open

Bdellovibrio attack-phase

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology
Researcher:
Sockett, Liz

Description

Raw data files of Bdellovibrio attack-phase

Files

keyimg_ycw2012-11-14-65.jpg
Files (18.8 MB)
Name Size
keyimg_ycw2012-11-14-65.jpg
md5:e1d594c3a28a65c3c097cef822fd3506
1.1 MB Preview Download
ycw2012-11-14-65_slicer14153.jpg
md5:e44dfcbcae59754ed9a9255ebda535ce
388.6 kB Preview Download
keymov_ycw2012-11-14-65.mp4
md5:c94dec22f96070f4666f2cb9d19cd44e
17.3 MB Download

Technical info

Tilt Series Date: 2012-11-14

Data Taken By: Yi-Wei Chang

Species / Specimen: Bdellovibrio bacteriovorus

Strain: HD100

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Strain from Liz Sockett

Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.

ycw2012-11-14-65_slicer14153.jpg: vesicle blebbing from cell . interesting spiral membrane vesicle thing

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2012-11-14-65/rawdata/

Bdellovibrio_wt_20121114_0030024.mrc, Tilt Series (Pixel Size 0.33 nm), 2.3 GB Download

Bdellovibrio_wt_20121114_0030024_full.rec, Reconstruction (Pixel Size 1.32 nm), 441.7 MB Download

Additional details

Identifiers Funding Dates
Created:
May 22, 2023
Modified:
August 28, 2025