Hippocampal Neurons (cultured)
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2014-08-21
Data Taken By: Matt Swulius
Species / Specimen: Rat Hippocampal Neuron (cultured)
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)
Purification / Growth Conditions / Treatment: Plunge frozen at DIV 14Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.
Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.
Attached Files
082014_neurons0017.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
082014_neurons0017_full.rec, Reconstruction (Pixel Size 2.4 nm), 712.7 MB
Files
keyimg_mst2014-08-21-10.jpg
Additional details
Identifiers
- DOI
- 10.22002/4gv9f-hep45
- TILT_SERIES_ID
- mst2014-08-21-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-08-21
- Created
-
2014-08-21
- Updated
-
2014-08-24