Published 2005 | Version v1
Dataset Open

Caulobacter crescentus

Creators

  • 1. ROR icon California Institute of Technology

Description

wild type CB15N. 2nd series of day; normalized after xrays erased, not reconstructed because alignment is bad. caulobacter 30 min post-synchrony; too close to focus by accident?

Technical info

Tilt Series Date: 2005-04-09

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 0.5°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -1.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft, Priism

Collaborators and Roles: specimen prep: DPD & AB, data collection & processing: D. Prabha Dias

Purification / Growth Conditions / Treatment: exponential phase, synchronized, 30 minutes post-synch, PYE media

Sample Preparation: box #430, grid #2; Quantifoil R2/2, 10nm gold, 100% humidity, -1 offset, 1 sec blot, 1 sec drain

Attached Files

CC05Apr09e200t02.mrc, Tilt Series (Pixel Size 1.2 nm), 2.0 GB

05Apr09t01b2n_full.rec, Reconstruction (Pixel Size 2.4 nm), 16.4 MB

Files

keyimg_pdd2005-04-09-2.jpg

Files (2.0 GB)

Name Size
16.4 MB (external) Download
2.0 GB (external) Download
md5:cdb9cdbad7102c34b960b66489a12192
48.5 kB Preview Download
md5:1d61752326ab29b4782581d7955b6ea3
1.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/4hpe1-nw733
TILT_SERIES_ID
pdd2005-04-09-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2005-04-09
Created
2008-06-18
Updated
2009-07-15