PAOI delta aer2 pJN105-aer2
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-02-12
Data Taken By: Ariane Briegel
Species / Specimen: Pseudomonas aeruginosa
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 10.0 μm, magnification: 34000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Kylie Watts (Loma Linda)
Purification / Growth Conditions / Treatment: induced pJN105-aer2, use 0.5% L-arabinoseStreak on LB plates and incubate overnight at 37°CInoculate 5 mL MOPS Minimal Media Limited Nitrogen (+ gentamicin for plasmid) from plate and grow at 30°C for ~24 hours
Attached Files
Pseudoaerplusinduced0002.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
Pseudoaerplusinduced0002_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB
Files
keyimg_aba2015-02-12-2.jpg
Additional details
Identifiers
- DOI
- 10.22002/4j7cz-b3012
- TILT_SERIES_ID
- aba2015-02-12-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-02-12
- Created
-
2015-02-12
- Updated
-
2015-02-25