Saccharomyces cerevisiae - JAWY1
Description
Technical info
Tilt Series Date: 2016-10-31
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.
Purification / Growth Conditions / Treatment: Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-10-31-4to5 (TS3,TS4)- Heat shocked @38 deg C for 2min10min. Other sm2016-10-31-* - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.
Sample Preparation: Final OD of 0.2 on grids (in growth medium) and blotted from behind
Attached Files
TS10_DB.rec, Reconstruction (Pixel Size 0.0 nm), 186.2 MB
TS10.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
Files
keyimg_smk2016-10-31-1.jpg
Files
(3.6 GB)
| Name | Size | |
|---|---|---|
|
md5:b74bcc738a791eebaff7130fafc17106
|
1.0 MB | Preview Download |
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md5:41d84c85bd15db6eba7d69931e01b2d1
|
7.2 MB | Preview Download |
| 3.4 GB | (external) Download | |
| 186.2 MB | (external) Download | |
|
md5:7039925b0c4b842df28f8f5e4b7768b1
|
386.3 kB | Preview Download |
|
md5:cdedaea03d365f943651fdd3ab129be6
|
378.8 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/4mm89-c9p09
- TILT_SERIES_ID
- smk2016-10-31-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-10-31
- Created
-
2017-01-16
- Updated
-
2017-01-25