Saccharomyces cerevisiae - JAWY1
Description
Technical info
Tilt Series Date: 2016-12-12
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: -100.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.
Purification / Growth Conditions / Treatment: Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. Heat shocked @38 deg C for 1hr50min and recovered @28deg C for 20 min (except sm2016-12-12-3 alias TS16 which was heat shocked for 1.5 hr and recovered for 40 min)
Attached Files
TS7.mrc, Tilt Series (Pixel Size 1.5 nm), 1.7 GB
TS7_4bin_NS_DB.rec, Reconstruction (Pixel Size 0.0 nm), 332.5 MB
Files
keyimg_smk2016-12-12-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/4n4s9-e2989
- TILT_SERIES_ID
- smk2016-12-12-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-12-12
- Created
-
2017-01-11
- Updated
-
2017-01-17