O. tauri G1

Description

Technical info

Tilt Series Date: 2009-12-10

Data Taken By: Lu Gan

Species / Specimen: Ostreococcus tauri

Strain: RCC745

Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 12h in 30uM Propyzamide, 3h Propyzamide washout

Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF

Attached Files

RCC745_G1tovis_306.mrc, Tilt Series (Pixel Size 0.96 nm), 755.0 MB

RCC745_G1tovis_306.rec, Reconstruction (Pixel Size 0.96 nm), 262.1 MB