Myxococcus xanthus PilP-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-08-44_slicer7203.jpg: tube ycw2014-03-08-44_slicer7204.jpg: ring ycw2014-03-08-44_slicer7210.jpg: vesicle ycw2014-03-08-44_slicer7211.jpg: external vesicles
Attached Files
20140308_YWC093_10045.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
20140308_YWC093_10045_full.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
Files
keyimg_ycw2014-03-08-44.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:a36d26697ac5d791cd2a0714006d1c35
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1.0 MB | Preview Download |
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md5:91c9b135cce742540c5d2d9d8bf0edbb
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md5:3e060263f56f1fc92d2a555d4ef1a099
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201.3 kB | Preview Download |
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md5:2631af9c9aa521497322ff434f970332
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md5:17a9af2d8c030fe0d2c254675c5c0664
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273.2 kB | Preview Download |
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md5:66d8300a09d370eeff2c805caa9dbe76
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240.3 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/4tas5-k3q18
- TILT_SERIES_ID
- ycw2014-03-08-44
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-08
- Created
-
2014-05-20
- Updated
-
2014-07-16