Published 2011
| Version v1
Dataset
Open
pLE7_1mM_IPTG
Description
These cells are highly overexpressing YFP-MreB from the pLE7 plasmid. These cells were a gift from Larry Rothfield.
Technical info
Tilt Series Date: 2011-11-18
Data Taken By: Matt Swulius
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: uploadraptor
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: LB 37 Degrees
Sample Preparation: Induced at an OD of .2 and grew for 2 hours (~OD of .8)
Attached Files
20111118_ple7_1mMiptg0022.mrc, Tilt Series (Pixel Size 0.95 nm), 1.1 GB
20111118_ple7_1mMiptg0022_full.rec, Reconstruction (Pixel Size 1.9 nm), 1.0 GB
Files
keyimg_mst2011-11-18-14.jpg
Additional details
Identifiers
- DOI
- 10.22002/4tb7q-n0092
- TILT_SERIES_ID
- mst2011-11-18-14
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-11-18
- Created
-
2011-11-22
- Updated
-
2011-11-23