Coxiella_ Verocells_28dpi

Description

Technical info

Tilt Series Date: 2017-09-25

Data Taken By: Debnath Ghosal

Species / Specimen: Coxiella burnetii

Strain: Nine Mile phase II clone 4

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.

Purification / Growth Conditions / Treatment: Refer to Heinzen lab.

Sample Preparation: Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.T

dga2017-09-25-70_slicer19011.jpg: multilayer structure beneath the inner membrane dga2017-09-25-70_slicer19012.jpg: straight structures extending outside the outer membrane

Attached Files

Cb_vero_201709250070.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB

Cb_vero_201709250070_part61_2.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB