Coxiella_burnetii_ wtACCMD_pH4d5

Description

Technical info

Tilt Series Date: 2017-08-15

Data Taken By: Debnath Ghosal

Species / Specimen: Coxiella burnetii

Strain: Nine Mile phase II clone 4

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.

Purification / Growth Conditions / Treatment: Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.

Sample Preparation: Refer to Heinzen lab. Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

dga2017-08-15-81_slicer18808.jpg: a discontinuity in the cell membrane dga2017-08-15-81_slicer18809.jpg: extracellular vesilce dga2017-08-15-81_slicer18810.jpg: strange shaped cell dga2017-08-15-81_slicer18951.jpg: filament in the cytoplasm?

Attached Files

Cb_20170817_Cb1pH4d5p0010.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB

Cb_20170817_Cb1pH4d5p0010_part61_6.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB