Cos7 CHAMP4B-PAGFP VPS4DN
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2015-01-24
Data Taken By: Cora Woodward
Species / Specimen: Cercopithecus aethiops (monkey, African green)
Strain: COS-7 ATCC CRL-1651
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with LipofectamineSample Preparation: manual blotting
Sample Preparation: manual blotting
Attached Files
15jan24_C204-1_po16_AT1BT1_0001.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
15jan24_C204-1_po16_AT1BT1_0001_full.rec, Reconstruction (Pixel Size 2.0 nm), 623.6 MB
Files
keyimg_cwl2015-01-24-7.jpg
Additional details
Identifiers
- DOI
- 10.22002/51ks1-x7p72
- TILT_SERIES_ID
- cwl2015-01-24-7
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-01-24
- Created
-
2015-01-25
- Updated
-
2019-03-07