Published 2014 | Version v1
Dataset Open

Vibrio deltaCheA2

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

V. cholerae strain deleted for CheA2

Technical info

Tilt Series Date: 2014-04-10

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

aba2014-04-10-13_slicer3828.jpg: polar array in delta CheA2 strain aba2014-04-10-13_slicer3829.jpg: PS of polar array in delta CheA2 strain

Attached Files

VibrioDeltaA20011.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioDeltaA20011_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

aba2014-04-10-13_slicer3828.jpg

Files (4.2 GB)

Name Size
md5:171b5472c18956b565bb83d7c4b1eb7b
149.7 kB Preview Download
md5:43e4aed7585f6dba013245a3001b5780
87.8 kB Preview Download
md5:692baea3b541bbb060e5833341710120
1.1 MB Preview Download
md5:6faad58332db6b36b57035ad732ddebc
27.1 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/535wk-1dc42
TILT_SERIES_ID
aba2014-04-10-13

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-10
Created
2014-04-10
Updated
2014-09-24