Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

immuno_excyto

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is an immuno labeled sample. The extracellular cytochrome supposed to be the nanowire is immuno labeled i.e. the primary antibody was against the extracellular cytochrome, and a gold conjugated secondary against the primary was used. This sample is fixed.

Technical info

Tilt Series Date: 2015-06-07

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand

Purification / Growth Conditions / Treatment: On imaging it is seen that there are way too many gold beads and also there is ice on the sample.

Sample Preparation: This is wt chemostat sample. Cu finder grids were used (the usual one). Blotting conditions- Blot force -6, blot time -3 , drain time - 1s.

Attached Files

immunoexcyto_manual1.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

immunoexcyto_manual1_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_psa2015-06-07-1.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:e0d780931cb921b9622ac95e0afb414f
969.5 kB Preview Download
md5:cfd0774e537a07dbb8de38ad370fe596
13.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/551k8-7mr36
TILT_SERIES_ID
psa2015-06-07-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-06-07
Created
2015-06-23
Updated
2015-07-01