O. tauri Propyzamide release
Description
Technical info
Tilt Series Date: 2010-02-12
Data Taken By: Lu Gan
Species / Specimen: Ostreococcus tauri
Strain: RCC745
Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan
Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide
Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF
lga2010-02-12-159_slicer7030.jpg: filament lga2010-02-12-159_slicer7031.jpg: filament
Attached Files
RCC745_RELtovis_171.mrc, Tilt Series (Pixel Size 1.26 nm), 562.0 MB
RCC745_RELtovis_171.rec, Reconstruction (Pixel Size 1.26 nm), 143.8 MB
Files
keyimg_lga2010-02-12-159.jpg
Files
(711.9 MB)
| Name | Size | |
|---|---|---|
|
md5:aeb412de9340389340121c98d78c0cef
|
891.5 kB | Preview Download |
|
md5:f8682a4ee3d8fac769d0067eefbbc465
|
4.7 MB | Preview Download |
|
md5:975de499cf9012aad7bf30037ef58525
|
183.5 kB | Preview Download |
|
md5:975de499cf9012aad7bf30037ef58525
|
183.5 kB | Preview Download |
| 562.0 MB | (external) Download | |
| 143.8 MB | (external) Download |
Additional details
Identifiers
- DOI
- 10.22002/57wym-qt943
- TILT_SERIES_ID
- lga2010-02-12-159
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-02-12
- Created
-
2010-05-12
- Updated
-
2014-07-16